protein marker promega broad range protein molecular weight markers; 10–245 kda  (Promega)

Journal: Biological Research

Article Title: Lead and calcium crosstalk tempted acrosome damage and hyperpolarization of spermatozoa: signaling and ultra-structural evidences

doi: 10.1186/s40659-024-00517-x

Figure Lengend Snippet: a Immunoblot showing the tyrosine phosphorylated protein (54 kDa) in sperm cells of the control and lead acetate (0.5–20 ppm) treated groups after 15 min of exposure. (LI—M.W marker L2—Control L3—0.5 ppm L4—5 ppm L5—10 ppm and L6—20 ppm). b : Immunoblot showing tyrosine phosphorylated proteins (54, and 30 KDa) in sperm cells of the control and lead acetate (0.5–20 ppm) treated groups after 3 h of exposure. (L1—M.W marker, L2—Control L3—0.5 ppm L4—5 ppm L5—10 ppm and L6—20 ppm). c Quantification of relative expression of tyrosine phosphorylated proteins (western blots) in sperm cells following in vitro exposure to different concentrations of lead acetate (0.5–20 ppm) for 15 min and 3 h

Article Snippet: Relative molecular weights of different protein bands were determined using broad-range molecular weight markers (pre-stained protein molecular weight, New England Biolabs) and gel documentation and analysis system.

Techniques: Western Blot, Control, Marker, Expressing, In Vitro